Cultivos de Tejidos y Banco Celular [TC]

REFERENCIA Nº: ATCC Nº: CRL-3242 (lote No 62363312) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Mus musculus, mouse , embryo

MORFOLOGÍA fibroblast; adherent

MEDIO DE CULTIVO: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. 

NUMERO DE PASE:

CARIOTIPO:

DNA PROFILE: STR-PCR Data:

PROCEDIMIENTO DE SUBCULTIVO: Note: Never allow culture to become completely confluent.

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels. 
   The recommended inoculum is 3 to 5 X 103 cells/cm2. Subculture before cultures become 80 to 90% confluent.

Interval: Every three days

Medium Renewal: 2 to 3 times per week

NIVEL DE BIOSEGURIDAD: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 1 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: CymaBay Therapeutics (formerly Metabolex, Inc.) & Choi Y

REFERENCIAS: Gregoire FM, et al. MBX-102/JNJ39659100, a novel peroxisome proliferator-activated receptor-ligand with weak transactivation activity retains antidiabetic properties in the absence of weight gain and edema. Mol. Endocrinol. 23(7): 975-88, 2009. PubMed: 19389808

COMENTARIOS:  The 3T3 L1-MBX clone was derived from 3T3-L1 (ATCC CL-173)to ensure close to 100% differentiation to adipocytes and great response to insulin. In addition, 3T3 L1-MBX has a great insulin-stimulated glucose uptake response which is about 8-10 fold window with sub-maximal insulin concentration in 2-deoxyglucose uptake assay (2-DOG). This cell line would be a valuable tool for researchers who are interested in diabetes and obesity research areas.

REFERENCIA Nº: ATCC Nº: CRL-3242 (lote No 62363312) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Mus musculus, mouse , embryo

MORFOLOGÍA fibroblast; adherent

MEDIO DE CULTIVO: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. 

NUMERO DE PASE:

CARIOTIPO:

DNA PROFILE: STR-PCR Data:

PROCEDIMIENTO DE SUBCULTIVO: Note: Never allow culture to become completely confluent.

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels. 
   The recommended inoculum is 3 to 5 X 103 cells/cm2. Subculture before cultures become 80 to 90% confluent.

Interval: Every three days

Medium Renewal: 2 to 3 times per week

NIVEL DE BIOSEGURIDAD: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 1 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: CymaBay Therapeutics (formerly Metabolex, Inc.) & Choi Y

REFERENCIAS: Gregoire FM, et al. MBX-102/JNJ39659100, a novel peroxisome proliferator-activated receptor-ligand with weak transactivation activity retains antidiabetic properties in the absence of weight gain and edema. Mol. Endocrinol. 23(7): 975-88, 2009. PubMed: 19389808

COMENTARIOS:  The 3T3 L1-MBX clone was derived from 3T3-L1 (ATCC CL-173)to ensure close to 100% differentiation to adipocytes and great response to insulin. In addition, 3T3 L1-MBX has a great insulin-stimulated glucose uptake response which is about 8-10 fold window with sub-maximal insulin concentration in 2-deoxyglucose uptake assay (2-DOG). This cell line would be a valuable tool for researchers who are interested in diabetes and obesity research areas.

REFERENCIA Nº: ECCC Nº: 05092802 (lote15A027) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Humano xenoinjerto de próstata

MORFOLOGÍA Epitelial. Agregados adherentes

MEDIO DE CULTIVO: RPMI 1640 sin rojo fenol (Sigma R7509) + 2 mM de glutamina + 10% suero bovino fetal (FBS)

NUMERO DE PASE: 7

CARIOTIPO: 49,XY,del(1)(p10),+i(1)(q10),der(2)t(2;4)(p13;q31)del(2)(q13q33),der(4)t(2;4)(p13;q31),t(6;14)(q15;q32),+7,+12[5]/50,idem,+3[1]

PERFIL DNA: STR-PCR de datos:

Amelogenina:X,Y
CSF1PO:10,11
D13S317:912
D5S818:11,12
D7S820:10,11
THO1:6,9.3
TPOX:8
vWA: 15,21

PROCEDIMIENTO DE SUBCULTIVO: Dividir los cultivos confluentes de 1:2 a 1.6 sembrando 2-4 x 10000 células/cm2 usando tripsina al 0.25% o EDTA/tripsina. Incubar a 37 C y 5% de CO2.

NIVEL DE BIOSEGURIDAD: 2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

DEPOSITOR: James W. JACOBBERGER, Case Western Reserve University, Cleveland, Ohio, EE.UU.

REFERENCIAS: Sramkoski et al . Una nueva línea celular de carcinoma de próstata humano, 22Rv1. In Vitro Cell. Dev. Biol. Anim. 35: 403 a 409, 1999.

COMENTARIOS: receptor de andrógenos positivo . 22Rv1 is a human prostate carcinoma epithelial cell line derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft. The cell line expresses prostate specific antigen (PSA). Growth is weakly stimulated by dihydroxytestosterone and lysates are immunoreactive with androgen receptor antibody by Western blot analysis. Growth is stimulated by epidermal growth factor (EGF) but is not inhibited by transforming growth factor beta-1 (TGF beta-1). This cell line is tumorigenic in nude mice.

REFERENCIA Nº: ECACC Nº: 85120602  (lote CB2737) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Human Embryo Kidney

MORFOLOGÍA: epitelial adherente

MEDIO DE CULTIVO: EMEM (EBSS) + 2 mM GLUTAMINA + 1% de aminoácidos no esenciales + 1mM Piruvato sódico + 10% Suero bovino fetal.

CARIOTIPO: 2n 46, hypotriploid, modal no. 64

Nº PASE: 66

PROCEDIMIENTO DE SUBCULTIVO: Dividir los cultivos subconfluentes en 1:3 a 1:6 sembrando de 2-4x10000 células/ml empleando tripsina al 0.25% o tripsina/EDTA. Se incuban a 37 C y 5% de CO2. Durante los subcultivos rutinarios las células deben siempre subcultivarse antes de alcanzar la confluencia. Estas células se despegan a temperatura ambiente. Pueden tardar hasta 7 días en adherirse. Cuando se descongelan, deben sembrarse en altas concentraciones.

NIVEL DE BIOSEGURIDAD: 1

REFERENCIAS: Virology 1977 77:319 PNAS USA 1996 93:4891 PNAS USA 1996 93:4192 Virology 1978 86:10 J Biol Chem

COMENTARIOS: Transformed with sheared human Ad5 DNA. Sensitive to human adenoviruses and adenovirus DNA. Can be used to isolate transformation defective host-range mutants of Ad5 and for titrating human adenoviruses. This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2%. The der (1)t(1;15) (q42;q13), der(19)t(3;19)(q12;q13),der(12)t(8;12) (q22;p13) and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+ and a single Xp+ in most cells. The Ad insert was shown to consist of a colinear segment from nucleotides 1 to 4344 integrated into chromosome 19 (19q13.2). Expression of an unusual cell surface receptor for vitronectin has been reported. This is composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit.

REFERENCIA Nº: ATCC: CRL-11268

SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas.

Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

The line is available with the following restriction: 1. The cell line was deposited at the ATCC by Rockefeller University and is provided for research purposes only. Neither the cell line nor the products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as a service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the cells, or their products, must first be negotiated with Rockefeller University, Office of Technology Transfer, 1230 York Avenue, New York, NY 10065 Attn: Kathleen A. Denis, Associate Vice President Technology Transfer.

DESCRIPCION CELULAR: Riñón de feto humano

MORFOLOGÍA: Epitelial y adherente

MEDIO DE CULTIVO: DMEM + 4 mM GLUTAMINA conteniendo 1.5 g/L de bicarbonato sódico y 4.5 g/L de glucosa + 10% Suero bovino fetal inactivado

NIVEL DE BIOSEGURIDAD: 2 [Cells contain Adeno and SV-40 viral DNA sequences]

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

CARIOTIPO: 2n=46

PROCEDIMIENTO DE SUBCULTIVO: Dividir los cultivos confluentes de 1:2 a 1.6 sembrando 2-4 x 10000 células/cm2 usando tripsina al 0.25% o EDTA/tripsina. Incubar a 37 C y 5% de CO2.

U.S. Patent Number:6,329,199

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.

REFERENCIAS: J. Nat. Cancer Inst. 1973; 51:1409.

COMENTARIOS: Es una línea derivada de la línea celular 293T. La línea 293T es una línea derivada de la 293 altamente transfectable en la que se ha insertado antígeno de SV40T. 293T fueron clonadas y co-transfectadas con los vectores pBND y pZAP para obtener la línea 293T/17 resistente. Estas células expresan el antígeno T del SV40 y el clon 17 es seleccionado por su alta transfectibilidad

293T/17 cells were cotransfected with the pCRIPenv- and the pCRIPgag-2 vectors to obtain the ANJOU 65 (see ATCC CRL-11269) cell line. ANJOU 65 cells were cotransfected with the pCRIPgag-2 and pGPT2E vectors to obtain the BOSC 23 (see ATCC CRL-11270) ecotropic envelope-expression packaging cell line. ANJOU 65 cells were also cotransfected with the pCRIPAMgag vector along with a plasmid expressing the gpt resistance gene to obtain the Bing (see ATCC CRL-11554) amphotropic envelope-expression packaging cell line

REFERENCIA Nº: ATCC Nº: CRL-1476 (lote CB No) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: aorta, thoracic/medial layer, Rattus norvegicus, rat, strain BDIX. embryo

MORFOLOGÍA myoblast , adherent

MEDIO DE CULTIVO: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. .

NUMERO DE PASE:

GENES EXPRESED: myokinase; creatine phosphokinase; myosin

CARIOTIPO:

DNA PROFILE: STR-PCR Data:

PROCEDIMIENTO DE SUBCULTIVO:

Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 3 to 4 days

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

NIVEL DE BIOSEGURIDAD: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: W Carlisle

REFERENCIAS: Kimes BW, Brandt BL. Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp. Cell Res. 98: 349-366, 1976. PubMed: 943301

Zhang X, et al. Microfilament depletion and circumvention of multiple drug resistance by sphinxolides. Cancer Res. 57: 3751-3758, 1997. PubMed: 9288783

Gordon EM, et al. Factor XII-induced mitogenesis is mediated via a distinct signal transduction pathway that activates a mitogen-activated protein kinase. Proc. Natl. Acad. Sci. USA 93: 2174-2179, 1996. PubMed: 8700904

Zhang X, Smith CD. Microtubule effects of welwistatin, a cyanobacterial indolinone that circumvents multiple drug resistance. Mol. Pharmacol. 49: 288-294, 1996. PubMed: 8632761

COMENTARIOS: The clonal cell line A10 was derived by B. Kimes and B. Brandt from the thoracic aorta of DBIX embryonic rat.

The clonal cell line A10 possesses many of the properties characteristic of smooth muscle cells.

The cells produce spontaneous action potentials at the stationary phase of the growth cycle and exhibit an increase in activity of the enzymes myokinase and creatine phosphokinase.

This cell line is a suitable transfection host.

REFERENCIA Nº: ATCC Nº: CRL-1619 (lote No70019044) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Homo sapiens, human, Skin, Malignant Melanoman,

MORFOLOGÍA: Adherent, epithelial

Tumorigenic

Yes;

Yes, in immunosuppressed mice

MEDIO DE CULTIVO: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. 37ºc

NUMERO DE PASE:

CARIOTIPO: It is a hypotriploid with a modal number of 62 chromosomes. There are 9 marker chromosomes that are commonly found in each cell, and normal N2, N6, and N22 are present at one copy per cell.

Mycoplasma contamination

Not detected

STR profiling

Amelogenin: X

CSF1PO: 11,12

D13S317: 11,14

D16S539: 9

D5S818: 12

D7S820: 9

THO1: 8

TPOX: 8,10

vWA: 16,17

PROCEDIMIENTO DE SUBCULTIVO: Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

NIVEL DE BIOSEGURIDAD: 1. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

DEPOSITOR: DJ Giard

REFERENCIAS: Consultar web ATCC

COMENTARIOS: This cell line is a suitable transfection host. This cell line is also the ideal control for NRAS mutant-A375 isogenic cell line (ATCC® CRL-1619IG-2™).

Female 54 years old.

APPLICATIONS:

3D cell culture

High-throughput screening

Toxicology

Immuno-oncology

REFERENCIA Nº: ATCC Nº: HTB-53(lote No 3531933) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Homo sapiens, human, pulmón, carcinoma

MORFOLOGÍA: epitelial, adherente

MEDIO DE CULTIVO: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

NUMERO DE PASE:

CARIOTIPO: at passage 60, hypotriploid to hypertriploid with abnormalities including dicentrics, minutes and large subtelocentric marker

TUMORIGENIC: Yes, in nude mice; forms an undifferentiated tumor suggestive of adenocarcinoma

DNA PROFILE: STR-PCR Data:

Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 11,12

D16S539: 11,13

D5S818: 12

D7S820: 8,12

THO1: 9

TPOX: 8,11

vWA: 17

ISOENZIMES:

AK-1, 2

ES-D, 1

G6PD, B

GLO-I, 1

PGM1, 1-2

PGM3, 1

PROCEDIMIENTO DE SUBCULTIVO: Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Interval: every 6 to 8 days

Subcultivation Ratio: 1:2 to 1:6

Medium Renewal: Twice per week

NIVEL DE BIOSEGURIDAD: 1. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: DJ Giard

SPECIAL COLLECTION: Human Tumor Cell Bank

REFERENCIAS: CONSULTAR LA WEB DE LA ATCC.

COMENTARIOS: The A-427 line was derived by D.J. Giard, as indicated in the description for ATCC HTB-41.

REFERENCIA Nº: ATCC Nº: CCL-185 (lote nº 3624224) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Carcinoma de pulmón humano

MORFOLOGÍA: epitelial adherente

MEDIO DE CULTIVO: Ham F12 modificado por Kaighn (F12K) con 2mM de glutamina modificado por la ATCC conteniendo 1.5 g/L de bicarbonato sódico +  10% Suero bovino fetal.

NUMERO DE PASE: 78

CARIOTIPO: This is a hypotriploid human cell line with the modal chromosome number of 66, occurring in 24% of cells. Cells with 64 (22%), 65, and 67 chromosome counts also occurred at relatively high frequencies; the rate with higher ploidies was low at 0.4%. There were 6 markers present in single copies in all cells. They include der(6)t(1;6) (q11;q27); ?del(6) (p23); del(11) (q21), del(2) (q11), M4 and M5. Most cells had two X and two Y chromosomes. However, one or both Y chromosomes were lost in 40% of 50 cells analyzed. Chromosomes N2 and N6 had single copies per cell; and N12 and N17 usually had 4 copies. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.

PROCEDIMIENTO DE SUBCULTIVO: Dividir los cultivos confluentes de 1:2 a 1.6 sembrando 2-4 x 10000 células/cm2 usando tripsina al 0.25% o EDTA/tripsina. Incubar a 37º C y 5% de CO2. Doubling time about 22 hours

NIVEL DE BIOSEGURIDAD: 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

DEPOSITOR: M Lieber

REFERENCIAS: Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758

Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365

Goodrum FD, Ornelles DA. The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. PubMed: 8985383

St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830

Horikami SM, et al. The Sendai virus V protein interacts with the NP protein to regulate viral genome RNA replication. Virology 222: 383-390, 1996. PubMed: 8806522

Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed:8676475

Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260

Fang R, Aust AE. Induction of ferritin synthesis in human lung epithelial cells treated with crocidolite asbestos. Arch. Biochem. Biophys. 340: 369-375, 1997. PubMed: 9143343

Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241

Evdokiou A, Cowled PA. Tumor-suppressive activity of the growth arrest-specific gene GAS1 in human tumor cell lines. Int. J. Cancer 75: 568-577, 1998. PubMed: 9466658

Giavedoni LD, Yilma T. Construction and characterization of replication-competent simian immunodeficiency virus vectors that express gamma interferon. J. Virol. 70: 2247-2251, 1996. PubMed: 8642649

Bartz SR, et al. Human immunodeficiency virus type 1 cell cycle control: Vpr is cytostatic and mediates G2 accumulation by a mechanism which differs from DNA damage checkpoint control. J. Virol. 70: 2324-2331, 1996. PubMed: 8642659

Garofalo R, et al. Transcriptional activation of the interleukin-8 gene by respiratory syncytial virus infection in alveolar epithelial cells: nuclear translocation of the RelA transcription factor as a mechanism producing airway mucosal inflammation. J. Virol. 70: 8773-8781, 1996. PubMed: 8971006

Jamaluddin M, et al. Inducible translational regulation of the NF-IL6 transcription factor by respiratory syncytial virus infection in pulmonary epithelial cells. J. Virol. 70: 1554-1563, 1996. PubMed: 8627674

Lewis JA, et al. Inhibition of mitochondrial function by interferon. J. Biol. Chem. 271: 13184-13190, 1996. PubMed: 8662694

Lieber M, et al. A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int. J. Cancer 17: 62-70, 1976. PubMed: 175022

COMENTARIOS: This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male. The cells are positive for keratin by immunoperoxidase staining. Studies by M. Lieber, et al. revealed that A549 cells could synthesize lecithin with a high percentage of desaturated fatty acids utilizing the cytidine diphosphocholine pathway.

REFERENCIA Nº: ECACC Nº: 93112517 (lote CB No 13J011) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Human ovarian carcinoma

MORFOLOGÍA Epithelial

MEDIO DE CULTIVO: RPMI 1640 + 2mM Glutamine + 1µM cisplatinum + 10% Foetal Bovine Serum (FBS)

NUMERO DE PASE: 5

CARIOTIPO: Modal no. 46

DNA PROFILE: STR-PCR Data:

Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 11,13
D5S818: 11
D7S820: 10
THO1: 6
TPOX: 8,10
vWA: 15,16

PROCEDIMIENTO DE SUBCULTIVO: Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 1x1,000 to 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells will attach slowly after resuscitation and take up to 7 days to reach confluency. Recommendation: resuscitate cells in media without cisplatin. Add after subculture of attached cells.

NIVEL DE BIOSEGURIDAD: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet

DEPOSITOR: Dr T H Ward, Cell Culture Unit, Patterson Laboratories, Christie Hospital, Manchester

REFERENCIAS: Cancer Res 1987;47:414; Cancer Res 1988;48:5713

COMENTARIOS: This cisplatin-resistant cell line has been developed by chronic exposure of the parent cisplatin-sensitive A2780 cell line (ECACC catalogue no. 93112519) to increasing concentrations of cisplatin. A2780cis is cross-resistant to melphalan, adriamycin and irradiation. An increased ability to repair DNA damage as well as cytogenetic abnormalities has been observed. In order to retain resistance cisplatinum has to be added to the media for every passage. In addition to this matched pair of drug-sensitive/resistant cell lines an adriamycin-resistant cell line, A2780adr (ECACC catalogue no. 93112520), has been isolated from the same parental line A2780

REFERENCIA Nº: ECACC Nº: 93112519   (lote 13J012) EN LAS  PUBLICACIONES CIENTÍFICAS DEBE IR CITADA COMO: A2780 (ECACC 93112519) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Human ovarian carcinoma

MORFOLOGÍA Epitelial. Adherente

MEDIO DE CULTIVO: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

NUMERO DE PASE: 14

CARIOTIPO: No especificado

DNA PROFILE: STR-PCR Data:

Amelogenin: X
CSF1PO: 10,11
D13S317: 12,13
D16S539: 11,13
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,10
vWA: 15,16

PROCEDIMIENTO DE SUBCULTIVO Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 3-6x10,000cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

NIVEL DE BIOSEGURIDAD: 2 Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens(UK).
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: Dr J Clarke, AVRI, Pirbright

REFERENCIAS: Semin Oncol 1984;11:285; Cancer Res 1987;47:414

Barretina J, et al., 2012 The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity. Nature. 483(7391):603-7. PMID: 22460905.

COMENTARIOS: The A2780 human ovarian cancer cell line was established from tumour tissue from an untreated patient. Cells grow as a monolayer and in suspension in spinner cultures. A2780 is the parent line to the cisplatin resistant cell line A2780 cis (ECACC catalogue no. 93112517) and the adriamycin resistant cell line A2780 ADR (ECACC catalogue no. 93112520).

REFERENCIA Nº: ECACC Nº: 90100401(lote 1436) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Larva de mosquito

MORFOLOGÍA: Epitelial

MEDIO DE CULTIVO: EMEM (EBSS) + 2 mM GLUTAMINA + 1% de aminoácidos no esenciales + 1mM Piruvato sódico + 20% Suero bovino fetal.

NUMERO DE PASE: 90

CARIOTIPO: 2n=6

PROCEDIMIENTO DE SUBCULTIVO: Dividir los cultivos subconfluentes en 1:3 a 1:6 sembrando de 2-4x10000 células/ml empleando tripsina al 0.25% o tripsina/EDTA. Se incuban a 28ºC y 5% de CO2. Durante los subcultivos rutinarios las células deben siempre subcultivarse antes de alcanzar la confluencia.

NIVEL DE BIOSEGURIDAD: 1

REFERENCIAS: Curr Sci. 1967; 36:506. Curr Tropics Microbiol. Immunol. 1971; 55:127

COMENTARIOS: La ATCC la denomina CCL 126. Deriva de un pool de larvas de Aedes albopictus. Las células son susceptibles a virus de mosquitos. Se emplean para estudios de virus

REFERENCIA Nº: ATCC Nº: HTB-111  (lote No62959340) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: uterus; endometrium, adenocarcinoma

MORFOLOGÍA epitelial

MEDIO DE CULTIVO: MEM + 2mM Glutamine + 1% NEAA +10% Foetal Bovine Serum (FBS).

NUMERO DE PASE:

CARIOTIPO:

DNA PROFILE: STR-PCR Data:

Amelogenin: X

CSF1PO: 13*

D13S317: 12,14

D16S539: 10,14

D5S818: 11,14

D7S820: 7*,10,7.1

THO1: 10,9.3*

TPOX: 8,10

vWA: 14,20

*Note: This cell line has historically exhibited instability at CSF1PO 13, D7S820 7, and THO1 9.3

ISOENZIMES:

AK-1, 1-2

ES-D, 1

G6PD, B

GLO-I, 2

PGM1, 1

PGM3, 1-2

PROCEDIMIENTO DE SUBCULTIVO: Dividir los cultivos confluentes de 1:2 a 1.6 sembrando 2-4 x 10000 células/cm2 usando tripsina al 0.25% o EDTA/tripsina. Incubar a 37 C y 5% de CO2.

NIVEL DE BIOSEGURIDAD: 1 Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens(UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: CJ Dawe

REFERENCIAS: Dawe CJ, et al. Growth in continuous culture, and in hamsters, of cells from a neoplasma assoicated with Acanthosis nigricans. J. Natl. Cancer Inst. 33: 441-456, 1964. PubMed: 14207855

Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

COMENTARIOS: The cells produce undifferentiated malignant tumors.

at low frequency (22%)

C. J. Dawe and associates derived this cell line from a metastatic lesion in the lymph node of a patient with endometrial carcinoma alerted to the condition by onset of the malignant disorder acanthosis nigricans

REFERENCIA Nº: ATCC Nº: CRL-1492  (lote  No 58231636) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Rat exocrine pancreatic tumour

MORFOLOGÍA Pancreas cells; Semi-adherent aggregates

MEDIO DE CULTIVO: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20% o RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

NUMERO DE PASE: 23

CARIOTIPO: Not specified

DNA PROFILE: STR-PCR Data:

RECEPTOR EXPRESION: insulin, expressed

glucocorticoid, expressed

CELLULAR PRODUCTS: amylase and other exocrine enzymes

PROCEDIMIENTO DE SUBCULTIVO: Cells grow in hollow spheroid colonies that can attach loosely. Maintain cultures between 5000-20000 cells/cm2; 5% CO2; 37°C. Cell viability may be poor on resuscitation from frozen (approx 50%) and growth can be slow. Cells may take up to 7 days to achieve 70% confluence. Resuscitate using 20% FBS, seeding at 10000 cells/cm2 and media change after 48 hours. Adherent cells should be removed using 0.05% Trypsin/EDTA. N.B. High cell viability cannot be expected during culture.

NIVEL DE BIOSEGURIDAD: 1. Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens(UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: NW Jessop

REFERENCIAS: Jessop NW, Hay RJ. Characteristics of two rat pancreatic exocrine cell lines derived from transplantable tumors. In Vitro 16: 212, 1980.

Longnecker DS, et al. Transplantation of azaserine-induced carcinomas of pancreas in rats. Cancer Lett. 7: 197-202, 1979. PubMed: 509403

Cockell M, et al. Identification of a cell-specific DNA-binding activity that interacts with a transcriptional activator of genes expressed in the acinar pancreas. Mol. Cell. Biol. 9: 2464-2476, 1989. PubMed: 2788241

Roux E, et al. The cell-specific transcription factor PTF1 contains two different subunits that interact with the DNA. Genes Dev. 3: 1613-1624, 1989. PubMed: 2612907

Seva C, et al. Lorglumide and loxiglumide inhibit gastrin-stimulated DNA synthesis in a rat tumoral acinar pancreatic cell line (AR42J). Cancer Res. 50: 5829-5833, 1990. PubMed: 2393852

Rajasekaran AK, et al. Structural reorganization of the rough endoplasmic reticulum without size expansion accounts for dexamethasone-induced secretory activity in AR42J cells. J. Cell Sci. 105: 333-345, 1993. PubMed: 7691838

Longnecker DS, et al. Effect of age on nodule induction by azaserine and DNA synthesis in rat pancreas. J. Natl. Cancer Inst. 58: 1769-1775, 1977. PubMed: 864754

Huang Y, Hui DY. Cholesterol esterase biosynthesis in rat pancreatic AR42J cells. Post- transcriptional activation by gastric hormones. J. Biol. Chem. 266: 6720-6725, 1991. PubMed: 2016288

Menniti FS, et al. Turnover of inositol polyphosphate pyrophosphates in pancreatoma cells. J. Biol. Chem. 268: 3850-3856, 1993. PubMed: 8382679

Logsdon CD, et al. Glucocorticoids increase amylase mRNA levels, secretory organelles, and secretion in pancreatic acinar AR42J cells. J. Cell Biol. 100: 1200-1208, 1985. PubMed: 2579957

Zhao H, et al. Regulation of intracellular Ca2+ oscillation in AR42J cells. J. Biol. Chem. 265: 20856-20862, 1990. PubMed: 1701171

Zhao H, Muallem S. Inhibition of inositol 1,4,5-trisphosphate-mediated Ca2+ release by Ca2+ in cells from peripheral tissues. J. Biol. Chem. 265: 21419-21422, 1990. PubMed: 2174872

Ihara H, Nakanishi S. Selective inhibition of expression of the substance P receptor mRNA in pancreatic acinar AR42J cells by glucocorticoids. J. Biol. Chem. 265: 22441-22445, 1990. PubMed: 1702421

Adell T, et al. Role of the basic helix-loop-helix transcription factor p48 in the differentiation phenotype of exocrine pancreas cancer cells. Cell Growth Differ. 11: 137-147, 2000. PubMed: 10768861

Seva C, et al. Growth-promoting effects of glycine-extended progastrin. Science 265: 410-412, 1994. PubMed: 8023165

Negre F, et al. Autocrine stimulation of AR4-2J rat pancreatic tumor cell growth by glycine-extended gastrin. Int. J. Cancer 66: 653-658, 1996. PubMed: 8647628

Bertrand V, et al. Inhibition of gastrin-induced proliferation of AR4-2J cells by calcium channel antagonists. Int. J. Cancer 56: 427-432, 1994. PubMed: 7508895

Mashima H, et al. Betacellulin and activin A coordinately convert amylase-secreting pancreatic AR42J cells into insulin-secreting cells. J. Clin. Invest. 97: 1647-1654, 1996. PubMed: 8601630

Palgi J, et al. Transcription factor expression and hormone production in pancreatic AR42J cells. Mol. Cell. Endocrinol. 165: 41-49, 2000. PubMed: 10940482

Mashima H, et al. Formation of insulin-producing cells from pancreatic acinar AR42J cells by hepatocyte growth factor. Endocrinology 137: 3969-3976, 1993. PubMed: 8756573

Silver K, Yao F. ARIP cells as a model for pancreatic beta cell growth and development. Pancreas 22: 141-147, 2001. PubMed: 11249068  

COMENTARIOS: Derived from a transplantable tumour of a rat exocrine pancreas. The line is tumourigenic in nude mice, and shows significant secretion of amylase and other exocrine enzymes. Secretory activity is inducible by glucocorticoid stimulation, and is accompanied by extensive re-organization of the endoplasmic reticulum. Secretory activity is inducible by glucocorticoid stimulation, and is accompanied by extensive re-organization of the endoplasmic reticulum.

REFERENCIA Nº: ATCC Nº: CRL-6322  (lote CB No) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Mus musculus, mouse, melanoma, C57BL/6J

MORFOLOGÍA mixture of spindle-shaped and epithelial-like cells; adherent

MEDIO DE CULTIVO: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

NUMERO DE PASE: 12

CARIOTIPO:

DNA PROFILE: STR-PCR Data:

PROCEDIMIENTO DE SUBCULTIVO:

 Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

1.- Remove and discard culture medium.

2.- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

5.- Add appropriate aliquots of the cell suspension to new culture vessels.

6.-Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days

NIVEL DE BIOSEGURIDAD: 1. Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens(UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR:

REFERENCIAS:

Fidler IJ. Biological behavior of malignant melanoma cells correlated to their survival in vivo. Cancer Res. 35: 218-224, 1975. PubMed: 1109790

Fidler IJ, et al. Tumoricidal properties of mouse macrophages activated with mediators from rat lymphocytes stimulated with concanavalin A. Cancer Res. 36: 3608-3615, 1976. PubMed: 953987

Fidler IJ, Bucana C. Mechanism of tumor cell resistance to lysis by syngeneic lymphocytes. Cancer Res. 37: 3945-3956, 1977. PubMed: 908034

Fidler IJ, Kripke ML. Metastasis results from preexisting variant cells within a malignant tumor. Science 197: 893-895, 1977. PubMed: 887927

Fidler IJ. Immune stimulation-inhibition of experimental cancer metastasis. Cancer Res. 34: 491-498, 1974. PubMed: 4812256

Briles EB, Kornfeld S. Isolation and metastatic properties of detachment variants of B16 melanoma cells. J. Natl. Cancer Inst. 60: 1217-1222, 1978. PubMed: 418183

Fidler IJ. Selection of successive tumour lines for metastasis. Nat. New Biol. 242: 148-149, 1973. PubMed: 4512654

COMENTARIOS: This cell line is a suitable transfection host.

REFERENCIA Nº: ATCC Nº: HTB-20 (lote No59758899) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: mammary gland; breast/duct

MORFOLOGÍA: Epitelial. adherent, patchy (The cells form adherent patches of epithelial-like cells The patches are compact multilayered colonies that rarely become confluent)  

MEDIO DE CULTIVO: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

NUMERO DE PASE: 92

CARIOTIPO: The cell line is aneuploid human female (XO usually), with most chromosome counts in the hypertetraploid range. Several chromosomes (N11, N13, and N22) are absent, and others are clearly under-represented (N9, N14, and N15) with respect to the other normal chromosomes. Chromosome N7 tends towards over-representation in several karyotypes. Some of the missing normal chromosomes are represented by their involvement in the nine stable marker chromosomes: der(14)t(14;?)(q32,?), unknown, iso(13q), der(6)t(6;7)(q21;q21), der(11)t(11;?)(14;?), del(11)(p11), unknown, unknown, der(2)t(2;?)(p21;?). Several of the latter were reported by E. Lasfargues, et al. Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572

DNA PROFILE: STR-PCR Data:

STR Profile

Amelogenin: X

CSF1PO: 10,11

D13S317: 11

D16S539: 9,11

D5S818: 11,13

D7S820: 9,12

THO1: 7

TPOX: 8

vWA: 15,16

 

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

Me-2, 0

PGM1, 1

PGM3, 1

PROCEDIMIENTO DE SUBCULTIVO: HTB-20 recovers slowly from cryopreservation. It may take two to four weeks for the cells to reach 70-80% confluence in a T-75 flask after thaw.

Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

NIVEL DE BIOSEGURIDAD: 1. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

EPOSITOR: EY Lasfargues

REFERENCIAS: Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572

Lasfargues EY, et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-729, 1979. PubMed: 94035

Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764

The cells form adherent patches of epithelial-like cells The patches are compact multilayered colonies that rarely become confluent

Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572

COMENTARIOS: The BT-474 line was isolated by E. Lasfargues and W.G. Coutinho from a solid, invasive ductal carcinoma of the breast of 60 years adult Caucasian female.

REFERENCIA Nº: ECACC Nº: 93120816  (LOTE 05H025) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Human primary pancreatic adenocarcinoma

MORFOLOGÍA: Epitelial, adherente

MEDIO DE CULTIVO: RPMI 1640 + 2mM Glutamine + 1mM Piruvato sódico + 10% Suero bovino fetal.

CARIOTIPO: 2n = 59, near triploid

PRODUCTOS: Mucina

Nº PASE: 38

PROCEDIMIENTO DE SUBCULTIVO: Dividir los cultivos subconfluentes en 1:3 a 1:6 sembrando de 2-4x10000 células/ml empleando tripsina al 0.25% o tripsina/EDTA. Se incuban a 37 C y 5% de CO2. Durante los subcultivos rutinarios las células deben siempre subcultivarse antes de alcanzar la confluencia.

NIVEL DE BIOSEGURIDAD: 1

REFERENCIAS: Clin Lab Med 1982;2:567; Cancer Invest 1986;4:15  

COMENTARIOS: Derived from a 61 year old female with a primary adenocarcinoma of the pancreas. BxPC-3 cells produce mucin and the tumour produced in a nude mouse is moderately well to poorly differentiated like the primary adenocarcinoma. Depositor: Dr M Ferrari, Instituto Zooprofilattico, Brescia

REFERENCIA Nº: ECACC Nº: 91031101 (lote No08F021) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Mouse C3H muscle myoblast

MORFOLOGÍA adherent

MEDIO DE CULTIVO: DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum (FBS).

NUMERO DE PASE: 22

CARIOTIPO:

DNA PROFILE: STR-PCR Data:

PROCEDIMIENTO DE SUBCULTIVO: Cells can be relatively slow growing when resuscitated from frozen taking 4-5 days to reach 50% confluence when seeded at 2x1,000 cells/cm² . Split semi-confluent cultures (50 - 70%) 1:3 to 1:6 i.e. seeding at 1-2x1,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Do not allow cells to reach confluence as myotube formation may occur.

NIVEL DE BIOSEGURIDAD: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: Obtained from ATCC

REFERENCIAS:

ature 1977;270:725, Science 1985;230;758-766, J. Cell Biol. 1994; 127:1755-1766[published erratum appears in J Cell Biol 1995 Feb;128(4):following 713], J.Virol. 1997;71:169-178, Proc. Natl. Acad.Sci. USA 1996;93:14082-14087, J. Biol. Chem. 1996;271:1386N

J Anim Sci; 1996 Jun: 74(6): 1265-73

COMENTARIOS: Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.

REFERENCIA Nº: ATCC Nº: CRL-10741(lote No 60208249) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

DESCRIPCION CELULAR: Human cells, Liver, Carcinoma; Hepatocellular

MORFOLOGÍA epitelial, Adherent

MEDIO DE CULTIVO: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

NUMERO DE PASE:

CARIOTIPO:

DNA PROFILE: STR-PCR Data:

Amelogenin: X,Y

CSF1PO: 10,11

D13S317: 9,13

D16S539: 12,13

D5S818: 11,13

D7S820: 10

THO1: 9

TPOX: 8,9

vWA: 17

Genes expressed:  alpha-fetoprotein (AFP, alpha fetoprotein); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin; alpha1 antichymotrypsin (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen, complement (C4); C3 activator; fibrinogen; alpha1 acid glycoprotein (alpha-1 acid glycoprotein); alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-binding protein)

PROCEDIMIENTO DE SUBCULTIVO:

 Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.

NIVEL DE BIOSEGURIDAD: 1. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: Baylor College of Medicine

PATENTS: 5,290,684

This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC.  As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.

REFERENCIAS: ver en web ATCC

COMENTARIOS: Male, White, 15 years.

This cell line is a suitable transfection host.

C3A is clonal derivative of Hep G2 that was selected for strong contact inhibition of growth, high albumin production, high production of alpha fetoprotein (AFP) and ability to grow in glucose deficient médium. As the cells become confluent, there is a marked reduction in AFP secretion and an increase in albumin secretion.

Gluconeogenesis activity is strongly oxgen dependent.

The cells have nitrogen metabolizing activity comparable to perfused rat livers.

There is no evidence of a Hepatitis B virus genome in this cell line.

REFERENCIA Nº: ECACC Nº: 86010202 lote (09I008) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: colon humano

MEDIO DE CULTIVO: EMEM (EBSS) + 2 mM GLUTAMINA + 1% de aminoácidos no esenciales + 1mM Piruvato sódico + 10% Suero bovino fetal.

NUMERO DE PASE: 52

CARIOTIPO: hipertetraploide

PROCEDIMIENTO DE SUBCULTIVO: Dividir los cultivos subconfluentes en 1:3 a 1:6 sembrando de 2-4x10000 células/ml empleando tripsina al 0.25% o tripsina/EDTA. Se incuban a 37 C y 5% de CO2. Durante los subcultivos rutinarios las células deben siempre subcultivarse antes de alcanzar la confluencia.

NIVEL DE BIOSEGURIDAD: 1

MORFOLOGÍA: Epitelial

REFERENCIAS: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 Adachi A, et al. Productive, persistent infection of human colorectal cell lines with human immunodeficiency virus. J. Virol. 61: 209-213, 1987. PubMed: 3640832 Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

COMENTARIOS: Se aisló de un tumor de colon primario de un hombre caucasiano de 72 años usando la técnica del explante.

Nº orden: 1061/2020

FECHA: 16/06/2020

REFERENCIA Nº: ATCC Nº: CRL-2095 (lote No58078592) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: tongue, human, squamous cell carcinoma

MORFOLOGÍA epitelial

MEDIO DE CULTIVO: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

NUMERO DE PASE:

CARIOTIPO: aneuploid; modal number = 43

DNA PROFILE: STR-PCR Data:

 Amelogenin: X

CSF1PO: 10,12

D13S317: 10,11

D16S539: 11,12

D5S818: 11,12

D7S820: 10

THO1: 6,9.3

TPOX: 8

vWA: 14,17

PROCEDIMIENTO DE SUBCULTIVO: Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, rinse and remove trypsin. Add fresh trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:6 is recommended

Medium Renewal: Every 2 to 3 days

NIVEL DE BIOSEGURIDAD: 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country..

DEPOSITOR: C Cardona

REFERENCIAS: Gioanni J, et al. Two new human tumor cell lines derived from squamous cell carcinomas of the tongue: establishment, characterization and response to cytotoxic treatment. Eur. J. Cancer Clin. Oncol. 24: 1445-1455, 1988. PubMed: 3181269

COMENTARIOS: Cal 27 was established in 1982 by J. Gioanni (Centre Antoine Lacassagne, Nice Cedex, France) from tissue taken prior to treatment from a 56 year old Caucasian male with a lesion of the middle of the tongue.

Solid tumors developed within 6 weeks in nude mice inoculated with 2 x 106 cells subcutaneously.

CAL 27 cells are epithelial, polygonal with a highly granular cytoplasm.

Immunocytochemical studies show strong positive staining with anti keratin antibodies.

The cells do not grow well in semi-solid medium.

Marked inhibition of thymidine incorporation was observed in the presence of VP16 (etoposide), CCNU (1-[2-chloroethyl]-3-cyclohexyl-1-nitrosourea), VM26 (teniposide), ADM (adriamycin), CPA (cyclophosphamide), and MTX (methotrexate).

CAL 27 cells were resistant to treatment with VDS (vindesine sulfate), CDP (cis-platinum) or ACTD (actinomycin D).

A culture submitted to the ATCC in December 1993 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.

REFERENCIA Nº:CCL-200 ATCC Nº: (lote No58078683) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Human, lung, carcinoma

MORFOLOGÍA: fibroblasto, adherente

MEDIO DE CULTIVO: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

NUMERO DE PASE: The cells senesce at about population doubling level (PDL) 19.

The current stock is at PDL 7.

CARIOTIPO: normal human male; diploid; stable

DNA PROFILE: STR-PCR Data:

ISOENZIMAS: G6PD, A

PROCEDIMIENTO DE SUBCULTIVO: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.

Subcultivation Ratio: 1:2 to 1:4
Medium Renewal: Every 2 to 3 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

NIVEL DE BIOSEGURIDAD: 1. ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

DEPOSITOR: MI Cour

REFERENCIAS:

COMENTARIOS: Derived from normal lung tissue from a patient with pancreatic carcinoma. Male. Black. 71 years

REFERENCIA: Nº ATCC: CRL-1459 (lote 63624239) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Colon humano normal

MORFOLOGÍA: fibroblasto

MEDIO DE CULTIVO: EMEM con 2mM glutamina + 1.5 g/ L bicarbonato sódico + 1 mM piruvato sódico + 0.1 mM de NEAA + 10% Suero bovino fetal. El medio está formulado para incubar a 37º C en atmósfera con 5% CO2 .También se puede usar el medio DMEM (glucosa 4,5 g/L) + 2 mM GLUTAMINA + 1mM Piruvato sódico + 10% Suero bovino fetal.

NUMERO DE PASE: 24. Adquiere la senescencia a partir del pase 42

CARIOTIPO:

PROCEDIMIENTO DE SUBCULTIVO: Dividir los cultivos confluentes de 1:2 a 1.6 sembrando 2-4 x 10000 células/cm2 usando tripsina al 0.25% o EDTA/tripsina. Incubar a 37º C y 5% de CO2.

NIVEL DE BIOSEGURIDAD: 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

DEPOSITOR: M. I. Cour

REFERENCIAS: Sugarman BJ , et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945,1985.PubMed:3933111
Hinterleitner TA , et al. Il-1 stimulates intestinal myofibroblast COX gene expression and augments activation of CI- secretion in T84 cells. Am. J. Physiol. 271: C1262-C1268, 1996. PubMed: 8897833

COMENTARIOS: Esta línea alcanza la senescencia a PDL=42. Su crecimiento se mejora mediante la adición de TNFalfa al medio

REFERENCIA Nº: ATCC Nº:CCL-215 (lote No3736181) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: human, lung, glioma.

MORFOLOGÍA : fibroblasto, adherente

MEDIO DE CULTIVO: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%

NUMERO DE PASE:

CARIOTIPO: normal male; diploid; stable

DNA PROFILE: STR-PCR Data:

Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 10,14

D16S539: 9,11

D5S818: 12

D7S820: 8,11

THO1: 6,7

TPOX: 11,12

vWA: 17,18

PROCEDIMIENTO DE SUBCULTIVO: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.

Subcultivation Ratio: 1:2 to 1:4
Medium Renewal: Every 2 to 3 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

NIVEL DE BIOSEGURIDAD: 1 ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitroge

DEPOSITOR: S Dilworth, 1977

REFERENCIAS:

COMENTARIOS: The line was established from the lung of a patient who died of glioma of the brain stem.

Male. White, 6 weeks.

REFERENCIA Nº: CRL-1790ATCC Nº: (lote No60018093)

SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Homo sapiens, human, Large intestine; Colon, Normal

MORFOLOGÍA epitelial, Adherent

MEDIO DE CULTIVO: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

NUMERO DE PASE:

CARIOTIPO: The line is diploid and no consistent marker chromosomes were observed.

DNA PROFILE: STR-PCR Data:

 Amelogenin: X

CSF1PO: 10,11

D13S317: 11,13

D16S539: 10,11

D5S818: 12,13

D7S820: 11

THO1: 7,8

TPOX: 9,10

vWA: 14,18

PROCEDIMIENTO DE SUBCULTIVO: Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant.
6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
7. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Twice per week

NIVEL DE BIOSEGURIDAD: 1. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: A Thompson

REFERENCIAS:

COMENTARIOS21 weeks gestation, Female. Morphologically the cells resemble epithelial cells; however, the cells do not contain keratin and definitive evidence of epithelial origin is lacking.

REFERENCIA Nº: ATCC Nº: CRL-2076 (LOTE 61923879) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: fibroblasto humano, piel

MORFOLOGÍA: fibroblasto

MEDIO DE CULTIVO: DMEM (EBSS) + 2 mM GLUTAMINA + 1mM Piruvato sódico + 10% Suero bovino fetal.

NUMERO DE PASE: 13

PROCEDIMIENTO DE SUBCULTIVO: Dividir los cultivos subconfluentes en 1:3 a 1:6 sembrando de 2-4x10000 células/ml empleando tripsina al 0.25% o tripsina/EDTA. Se incuban a 37 C y 5% de CO2. Durante los subcultivos rutinarios las células deben siempre subcultivarse antes de alcanzar la confluencia.

NIVEL DE BIOSEGURIDAD: 1

REFERENCIAS:

COMENTARIOS: The line was established from skin taken from normal foreskin. The cells are capable of approximately 54 population doublings before the onset of senescence.

REFERENCIA Nº: ECACC Nº: 85050302 (lote CB No1824) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Hamster Chinese ovary

MORFOLOGÍA Epithelial; Adherent

MEDIO DE CULTIVO: Ham's F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

NUMERO DE PASE:

CARIOTIPO: Hypodiploid, modal no. 20

DNA PROFILE: STR-PCR Data:

PROCEDIMIENTO DE SUBCULTIVO: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

NIVEL DE BIOSEGURIDAD: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens(UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: Dr R Downing, PHLS CAMR, Porton Down, Salisbury

REFERENCIAS: J Exp Med 1958;108:945

COMENTARIOS: A cell line originally derived from Chinese Hamster Ovary cells by Puck in 1957. The cells have an absolute requirement for L-proline

REFERENCIA Nº: ECACC Nº: 91041114 (lote CB No00F031) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Salmon embryo, Fish

MORFOLOGÍA Adherent

MEDIO DE CULTIVO: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

NUMERO DE PASE:

CARIOTIPO: Not specified

DNA PROFILE: STR-PCR Data:

PROCEDIMIENTO DE SUBCULTIVO: Split sub-confluent cultures (70-80%) 1:3 to 1:6; i.e seeding at 1-4 x 10,000 cells per cm using 0.25% trypsin or trypsin/EDTA, cells detach after 5-10 minutes at room temperature. Grow cells in dark (e.g. wrapped in foil); 5% CO2; 21°C.

Freeze cells in 5% DMSO and 95% foetal bovine serum (FBS). Fish cell lines detach easily during transit if the culture is too young. For resuscitation seed flasks at 4-5 x 10,000 cells per cm and then split cells 1:3 to 1:4 and culture for at least one week with 1-2 media changes before shipping.

NIVEL DE BIOSEGURIDAD: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens(UK).
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: Obtained from ATCC

PATENTES: None specified by Depositor

REFERENCIAS: Ann NY Acad Sci 1965;126:566-586In Vitro 1984;20:671-676

COMENTARIOS: Derived from a Chinook salmon (Oncorhynchus tshawytscha) embryo, susceptible to a wide range of fish viruses and in many instances replicate high titres.

REFERENCIA Nº: ECACC Nº89111413.  SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: carcinoma de recto de ratón

MORFOLOGÍA: epitelial adherente

MEDIO DE CULTIVO: DMEM (EBSS) + 2 mM GLUTAMINA  + 1mM Piruvato sódico + 10% Suero bovino fetal.

NUMERO DE PASE:

CARIOTIPO: hiperdiploide n 50

PROCEDIMIENTO DE SUBCULTIVO: Dividir los cultivos subconfluentes en 1:3 a 1:6 sembrando de 2-4x10000 células/ml empleando tripsina al 0.25% o tripsina/EDTA. Se incuban a 37 C y 5% de CO2. Durante los subcultivos rutinarios las células deben siempre subcultivarse antes de alcanzar la confluencia.

NIVEL DE BIOSEGURIDAD: 1

REFERENCIAS: J Pathol 1978;124:35

COMENTARIOS: Procedente de un ratón C57BL/1CRF macho de 19 meses de edad, que había recibido una inyección de MAMA, cada semana durante 18 meses. Las células producen grandes tumores en ratones desnudos a partir del mes.

REFERENCIA Nº: ATCC Nº: CRL-2638 (LOTE 61559123) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Mus musculus, mouse colon carcinoma

MORFOLOGÍA: fibroblasto, adherente

MEDIO DE CULTIVO: RPMI 1640 + 2 mM GLUTAMINA + 1mM Piruvato sódico + 10% Suero bovino fetal.

TUMORIGENICA: si

EFECTOS: in BALB/c mice. Mice inoculated, subcutaneously, developed lethal tumors at 80% frequency with 10(3) cells and at 100% with 10(4) cells. Pulmonary metastases developed when mice were inoculated, intravenously, with 10(4) cells

Antigen Expression: H-2d

PROCEDIMIENTO DE SUBCULTIVO: Dividir los cultivos subconfluentes en 1:3 a 1:6 sembrando de 2-4x10000 células/ml empleando tripsina al 0.25% o tripsina/EDTA. Se incuban a 37 C y 5% de CO2. Durante los subcultivos rutinarios las células deben siempre subcultivarse antes de alcanzar la confluencia.

NIVEL DE BIOSEGURIDAD: 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: N Restifo

REFERENCIAS: Wang M, et al. Active immunotherapy of cancer with a nonreplicating recombinant fowlpox virus encoding a model tumor-associated antigen. J. Immunol. 154: 4685-4692, 1995. PubMed: 7722321  

COMENTARIOS: CT26 is an N-nitroso-N-methylurethane-(NNMU) induced, undifferentiated colon carcinoma cell line. It was cloned to generate the cell line designated CT26.WT (ATCC CRL-2638). The cell line can be used with CT26.CL25 (ATCC CRL-2639) as a model for testing immunotherapy protocols and in studies on the host immune response. CT26.WT was stably transduced with the retroviral vector LXSN that contains the lacZ gene encoding the model tumor associated antigen (TAA), beta-galactosidase (beta-gal) to obtain the lethal subclone CT26.CL25 (ATCC CRL-2639).

The growth rate and lethality of CT26.CL25 and CT26.WT is virtually identical despite the expression by CT26.CL25 of the model TAA, beta-galactosidase, in normal mice.

A culture submitted to the ATCC in July of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.

The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative

REFERENCIA Nº: ATCC Nº: HTB-185(lote No 2856937) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Homo sapiens, human, Medulloblastoma, Brain; Cerebellum

MORFOLOGÍA Mixed: multicellular aggregates in suspension and some adherent cells, epitelial,

MEDIO DE CULTIVO: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

NUMERO DE PASE:

CARIOTIPO: The karyotype is 45, XY, -7, -8, -17, -20, der(20)t(1;20)(q12;q13), 8q+, 17p+ (range = 41 to 46). This is a hypodiploid cell line with a frequency of higher ploidies of 5.4%. Three marker chromosomes are present in all cells. They are: der(20)t(1;20)(q12;q13), 8q+ and 17p+. N7, N17 and N20 have single copies. The single X is structurally normal, and the Y chromosome is present as confirmed by fluorescence microscopy.

DNA PROFILE: STR-PCR Data:

Amelogenin: X,Y

CSF1PO: 9,12

D13S317: 8,10

D16S539: 11

D5S818: 11

D7S820: 10

THO1: 7

TPOX: 8,11

vWA: 16,18

ISOENZYMES:

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 0

PGM1, 1

PGM3, 1

PROCEDIMIENTO DE SUBCULTIVO: Cultures can be maintained by addition or replacement of medium. Adherent cells can be dislodged by scraping, and cultures can be established by resuspending a cell pellet at 5 X 105viable cells/mL. Maintain cultures between 4 X 104 and 8 X 105 cells/mL.

Medium Renewal: 2 to 3 times per week

NIVEL DE BIOSEGURIDAD: 1. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

GENES EXPRESSED: The cells produce tumors in nude mice and the resulting tumors are glutamine synthetase positive; neuron specific enolase positive; glial fibrillary acidic proteins negative; S100 (S-100) protein negative; The cells express elevated levels of four biochemical markers of SCLC (neuron specific enolase; the brain isoenzyme of creatine kinase; L-DOPA decarboxylase; bombesin-like immunoreactivity

DEPOSITOR: HS Friedman

REFERENCIAS: Consultar web ATCC

COMENTARIOS: The D283 Med cell line was established in 1985 by Friedman et al. from malignant ascites cells and a peritoneal metastasis from a boy with medulloblastoma. ­

Derived from metastatic site, peritoneum.

The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein.

REFERENCIA Nº: ATCC Nº: HTB-186 (lote No2056451) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Brain; Cerebellum, Homo sapiens, human, Desmoplastic Cerebellar Medulloblastoma

MORFOLOGÍA:  Adherent, polygonal

MEDIO DE CULTIVO: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

NUMERO DE PASE:

CARIOTIPO: This is a hypertetraploid human cell line with a modal number between 93 and 99. The frequency of cells with higher ploidies is 2.0%. However, since the stemline chromosome number is high, the estimate for the polyploidy is tentative. Thirteen or more marker chromosomes were common to all cells. Of these, many had two to four copies per cell. Among the markers were: t(1q5q), t(13q;?), 15p+, 7q+, der(9)t(3;9)(p21;q34) and eight others. In most cells, the 15p+ has three copies and der(9) has four copies.Some cells have del(1)(p11). Normal N12, N14, N15 and N19 tend to have four or more copies per cell. There are two normal X chromosomes in most cells, but there is no detectable normal Y.

DNA PROFILE: STR-PCR Data:

Amelogenin: X

CSF1PO: 11

D13S317: 13,14

D16S539: 10

D5S818: 11,13

D7S820: 8,10

THO1: 9

TPOX: 8,10

vWA: 14,20

ISOENZYMES:

AK-1, 1

ES-D, 1-2

G6PD, B

GLO-I, 1

Me-2, 1

PGM1, 2

PGM3, 1-2

PROCEDIMIENTO DE SUBCULTIVO: Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C, 5% CO2

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Population doublung time: Approximately 34 hrs

NIVEL DE BIOSEGURIDAD: 1. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

DEPOSITOR: HS Friedman

SPECIAL COLLECTION: Human Tumor Cell Bank

REFERENCIAS: Consultar la web de la ATCC

COMENTARIOS: This cell line is a suitable transfection host. The Daoy cell line was established in 1985 by P. F Jacobsen of the Royal Perth Hospital in Western Australia.

The line was derived from biopsy material taken from a tumor in the posterior fossa of a 4 year old boy.

Although the original tumor had characteristics of both neuronal and glial differentiation, these were not retained by the cell line.

Treatment of the cells with dibutyryl cyclic amp (cAMP) does not induce expression of those characteristics as measured by staining for S100 (S-100) protein and glial fibrillary acidic proteins (GFAP).

REFERENCIA Nº: ECACC Nº: 94062922 (lote No99G031) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

PATENTES: This material is cited in a US and/or other Patent and may not be used to infringe parent claims. US Patent No. 5,192,679

DESCRIPCION CELULAR: Canine Monocyte-macrophage

MORFOLOGÍA Macrophage

MEDIO DE CULTIVO: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).

NUMERO DE PASE:

CARIOTIPO: Not specified

DNA PROFILE: STR-PCR Data:

PROCEDIMIENTO DE SUBCULTIVO: Cells are semi-adherent, i.e. some cells grows in suspension, whilst most attach to the surface and may flatten. Attached cells should be removed with 0.02% EDTA. Split sub-confluent cultures (70-80%)  i.e. seeding at 2-4x10,000 cells/cm²; 5% CO2; 37°C.  

NIVEL DE BIOSEGURIDAD: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens(UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: Obtained from ATCC

REFERENCIAS: Wellman ML, Krakowka S, Jacobs RM, Kociba GJ 1988 A macrophage-monocyte cell line from a dog with malignant histiocytosis. In Vitro Cell Dev Biol.24(3):223-9. PMID: 3350786

Gröne A, Fonfara S, Baumgärtner W. 2002 Cell type-dependent cytokine expression after canine distemper virus infection. Viral Immunol. 15(3):493-505. PMID: 12479398

COMENTARIOS: Derived from a ten year old male golden retriever with malignant histiocytosis. The cells have a macrophage-like morphology and are able to phagocytose latex particles. They are positive for Fc-gamma receptors and are negative for Fc-mu and C3b receptors. DH82 cells do not produce IL-1

REFERENCIA Nº: ECACC Nº: 85023105  SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Mouse ascites lymphoma lymphoblast

MORFOLOGÍA: Lymphoblast

MEDIO DE CULTIVO: DMEM (EBSS) + 2 mM GLUTAMINA + 1mM Piruvato sódico + 10% Suero bovino fetal.

CARIOTIPO: 2n = 39

PROCEDIMIENTO DE SUBCULTIVO: Cuando se descongelan, hay que retirar la solucion criopreservante mediante centrifugación a 100 g durante 5 minutos. Mantener los cultivos entre 3-9x100,00 células/mL. Se incuban a 37 C y 5% de CO2.

NIVEL DE BIOSEGURIDAD: 1

PRODUCTOS:  A surface antigen induced by leukaemia type G virus; H-2b and Thy-1.2 antigens; Interleukin 4 (IL-4), H-2b, Thy1.2

DEPOSITOR: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford

REFERENCIAS: Br J Cancer 1950;4:372; Cancer Res 1965;25:813; J Immunol 1972;108:1146; J Nat Cancer Inst 1972;48:265

BIBLIOGRAFÍA ADICIONAL EN LA WEB DE LA ECACC

COMENTARIOS: Established from a lymphoma induced in a C57BL/6N mouse by 9,10-dimethyl-1,2-benzanthracene. The cells have been reported to produce low levels of type G (Gross) virus antigen (J Nat Cancer Inst 1972;48:265) H-2b, IL-4 and Thy-1.2 antigen. The cell line is resistant to cortisol and dexamethasone and is sensitive to PHA

REFERENCIA Nº: ATCC Nº: CRL-1423 (lote 7390625) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: osteosarcoma, hueso, humano

MORFOLOGÍA: fibroblasto y adherente

MEDIO DE CULTIVO: McCoy's 5a Medium Modified  + 10% Suero bovino fetal

NUMERO DE PASE: 15

CARIOTIPO:

ISOENZIMAS: G6PD, B

PROCEDIMIENTO DE SUBCULTIVO: Dividir los cultivos confluentes de 1:2 a 1.6 sembrando 2-4 x 10000 células/cm2 usando tripsina al 0.25% o EDTA/tripsina. Incubar a 37 C y 5% de CO2.

NIVEL DE BIOSEGURIDAD: 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

DEPOSITOR: PT Peeble

REFERENCIAS: Peebles P, et al. Isolation of four unusual pediatric solid tumor cell lines. Pediatr. Res. 12: 485, 1978.

Zhang W, et al. EGF-mediated phosphorylation of extracellular signal-regulated kinases in osteoblastic cells. J. Cell. Physiol. 162: 348-358, 1995. PubMed: 7860643

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

COMENTARIOS: osteosarcoma de mujer caucasiana de 9 años

REFERENCIA Nº: ECACC Nº: 88030401 (lote 02AO69) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Human Caucasian malignant melanoma

MORFOLOGÍA: epitelial adherente

MEDIO DE CULTIVO: McCoy's 5a Medium Modified  + 10% Suero bovino fetal

NUMERO DE PASE: 17

CARIOTIPO: Triploid, modal n.o 69

PRODUCTOS: Melanina

PROCEDIMIENTO DE SUBCULTIVO: Dividir los cultivos sub-confluentes de 1:2 a 1.6 sembrando 2-4 x 10000 células/cm2 usando tripsina al 0.25% o EDTA/tripsina. Incubar a 37 C y 5% de CO2.Las células tardan en adherirse.

NIVEL DE BIOSEGURIDAD: 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

DEPOSITOR:

REFERENCIAS: Paediatric Res 1978;12:485

COMENTARIOS: Established from a malignant melanoma of a 31 year old male Caucasian. The cells produce melanin for up to 50 population doublings. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis

REFERENCIA Nº: ATCC Nº: HTB-148 (lote No70039817) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Homo sapiens, human, Brain, neuroglioma

MORFOLOGÍA epitelial, Adherent

MEDIO DE CULTIVO: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

NUMERO DE PASE:

CARIOTIPO:

Modal number = 73; range = 63 to 78.
This is a hypertriploid human cell line having the modal chromosome number of 73 occurring in 26% of cells. However, cells having 75 chromosomes also occurred at a high rate (24%). Higher ploidies were found at 0.4%. At least 16 marker chromosomes are common to all metaphases examined: paired del(2)(p2209) and del(9)(p22) and single der(14)t(1;14)(p22;q22), del(3)(p2501), del(7)(q3209), del(10)(p1301), t(13q17q), t(3p13q) and at least eight others. The del(5) (p13), del(7) (q11) and a few others occurred in some, and many others were seen only once. N7 and N21 occurred in 4 or more copies per cell. Most cells had two X and two Y chromosomes

Mycoplasma contamination: Not detected

STR profiling

Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 12

D16S539: 11,12

D5S818: 10,12

D7S820: 8,11

THO1: 7,9

TPOX: 8,11

vWA: 14,18

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 0

PGM1, 1-2

PGM3, 1

Tumorigenic: No;No, in immunosuppressed mice

Yes, in semisolid medium

PROCEDIMIENTO DE SUBCULTIVO: Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:15 is recommended
Medium Renewal: 2 to 3 times per week

Reagents for cryopreservation

Complete growth medium supplemented with 5% (v/v) DMSO

NIVEL DE BIOSEGURIDAD: 1. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: J Riggs

Human Tumor Cell Bank

REFERENCIAS:

COMENTARIOS This cell line is a suitable transfection host

REFERENCIA Nº: ATCC Nº: CRL-1446  (lote date frozen09/09/03) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Rattus norvegicus, rat , heart/myocardium

MORFOLOGÍA mioblasto

MEDIO DE CULTIVO: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

NUMERO DE PASE:

RECEPTOR EXPRESION: acetylcholine, expressed

PRODUCTOS CELULARES: myokinase; creatine phosphokinase; myosin

GENES EXPRESADOS: myokinase; creatine phosphokinase; myosin

PROCEDIMIENTO DE SUBCULTIVO: The myoblastic population will become depleted rapidly if the cultures are allowed to become confluent.
To prevent loss of myoblastic cells, cultures should be subcultured before they become confluent, and the line should be recloned periodically with selection for myoblastic cells.

Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

NIVEL DE BIOSEGURIDAD: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: W Carlisle

REFERENCIAS: Kimes BW, Brandt BL. Properties of a clonal muscle cell line from rat heart. Exp. Cell Res. 98: 367-381, 1976. PubMed: 943302

Levy AP, et al. Post-transcriptional regulation of vascular endothelial growth factor by hypoxia. J. Biol. Chem. 271: 2746-2753, 1996. PubMed: 8576250

COMENTARIOS: H9c2(2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue by B. Kimes and B. Brandt and exhibits many of the properties of skeletal muscle This cell line is a suitable transfection host. Myoblastic cells in this line will fuse to form multinucleated myotubes and respond to acetylcholine stimulation.

Fusion occurs faster if the serum concentration in the medium is reduced to one percent

REFERENCIA Nº: ATCC Nº: HTB: 119 (lote No70018326) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: carcinoma; small cell lung cancer; male; human; Caucasian

MORFOLOGÍA: suspension, multicell aggregates; floating aggregates

MEDIO DE CULTIVO: El medio base recomendado por la ATCC es RPMI-1640 Medium, (ATCC 30-2001). Para realizar el medio completo hay que adicionar al medio: fetal bovine serum (ATCC 30-2020) hasta una concentración final de 10%. Si se usa un medio con otra referencia, hay que comprobar que la composición es la misma (concentración de glucosa, piruvato sódico…)

NUMERO DE PASE:

CARIOTIPO:modalnumber=76to78;range=40to87
This is an aneuploid human male cell line. Monosomy of many of the normal chromosomes is noted as well as bisomy in this subtetraploid cell line; however, translocations and deletions involving many of the missing chromosomes are noted, and these chromosomal rearrangements appear to be stable and generally paired. Twelve marker chromosomes were identified including: der(16)t(1;16)(q21;q23), der(22)t(4;22)(q12;q13), der(12)t(11;12)(q23;p12), del(17)(p11), der(19)t(5;19)(?q21;q13) and others.

DNA PROFILE: STR-PCR Data:

CSF1PO: 10, 12

D13S317: 12

D16S539: 11

D5S818: 11, 13

D7S820: 9

TH01: 8, 9

TPOX: 10

vWA: 16, 17

Amelogenin: XY

ISOENZYMES:

AK-1, 1

ES-D, 2

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 2

PGM3, 1

GENES EXPRESSED: Oncogenes: myc +; myb +; fes +; fms +; raf +; ras +

RECEPTOR EXPRESION: insulin-like growth factor II (IGF II)

PROCEDIMIENTO DE SUBCULTIVO: Attached cells can be detached by shaking flask Allow aggregates to settle to the bottom of the flask, remove and discard the supernatant medium. Add fresh medium, disperse cells by gentle pipetting and dispense into new flasks. Do not break down aggregates. Subculture every 6 to 8 days.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 times weekly

NIVEL DE BIOSEGURIDAD: 1. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: AF Gazdar

Deposited AsHomo sapiens

REFERENCIAS: Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed: 6646201

Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917

Schardt C, et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141

Broers JL, et al. Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines. J. Cell Sci. 91: 91-108, 1988. PubMed: 2473086

Rygaard K, et al. Expression of myc family oncoproteins in small-cell lung-cancer cell lines and xenografts. Int. J. Cancer 54: 144-152, 1993. PubMed: 8386707

Gazdar AF, et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156

Adi F, et al. Establishment of Continuous, Clonable Cultures of Small-Cell Carcinoma of the Lung Which Have Amine Precursor Uptake and Decarboxylation Cell Properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156

Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

Gazdar AF, et al. Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties. Cancer Res. 45: 2924-2930, 1985. PubMed: 2985258

Kiefer PE, et al. Amplification and expression of protooncogenes in human small cell lung cancer cell lines. Cancer Res. 47: 6236-6242, 1987. PubMed: 2824028

Hensel CH, et al. Altered structure and expression of the human retinoblastoma susceptibility gene in small cell lung cancer. Cancer Res. 50: 3067-3072, 1990. PubMed: 2159370

Lung Cancer 4: 155-161, 1988.

Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760

Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241

The cells form tumors with typical small cell carcinoma histology.

COMENTARIOS This cell line is aneuploid, will form colonies in soft agar and retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics.

The cells grow in aggregates, thus cell counts are not accurate.

The cells stain positively for cytokeratins.

The line can be adapted to grow in shaker flask or spinner flask systems.

The N-myc gene is amplified, and there is expression of the mRNA and protein.

C-myc mRNA, but not protein, is expressed at a low level.

There is expression of c-myb, v-fes, v-fms, c-raf 1, Ha-ras, Ki-ras and N-ras mRNA.

The cells form tumors with typical small cell carcinoma histology.)

Yes, forms colonies in soft agar

REFERENCIA Nº: ECACC: 90032006 (lote No CB2014) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Human ileocecal adenocarcinoma

MORFOLOGÍA Epithelial, adherent

MEDIO DE CULTIVO: RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS) or 5% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS).

NUMERO DE PASE: 21

CARIOTIPO: 2n = 46, pseudodiploid

DNA PROFILE: STR-PCR Data:

Amelogenin: X,Y

CSF1PO: 12

D13S317: 8,11

D16S539: 12,13

D5S818: 13

D7S820: 10,12,11.3

TH01: 7,9.3

TPOX: 8,11

vWA: 18,19

Isoenzymes

AK-1, 1

ES-D, 1-2

G6PD, B

GLO-I, 2

Me-2, 1

PGM1, 1

PGM3, 

GENES EXPRESSED:  carcinoembryonic antigen (CEA) 0.5 ng/106 cells/10 days; alkaline phosphatase. The cells are positive for keratin by immunoperoxidase staining.

CELLULAR PRODUCTS: carcinoembryonic antigen (CEA) 0.5 ng/10 exp6 cells/10 days; alkaline phosphatase; keratin

TUMORIGENIC: yes Yes, in nude mice

EFFECTS: Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells

PROCEDIMIENTO DE SUBCULTIVO: Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C.

NIVEL DE BIOSEGURIDAD Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: Obtenined from ATCC

REFERENCIAS:

Tompkins WA, Watrach AM, Schmale JD, Schultz RM, Harris JA 1974 Cultural and antigenic properties of newly established cell strains derived from adenocarcinomas of the human colon and rectum. J Natl Cancer Inst. 52(4):1101-10 PMID: 4826581.

Nelson-Rees WA, Flandermeyer RR, Hawthorne PK 1975 Distinctive banded marker chromosomes of human tumor cell lines. Int J Cancer. 16(1):74-82 PMID: 1058173.

PATENTS: None specified by Depositor

COMENTARIOS From a 67 year old male.

The four cell lines: DLD-1 (90102540), HCT-15 (91030712), HCT-8 (90032006) and HRT-18 (86040306) have been shown to have a common genetic origin see Vermeulen SJ, Chen TR, Speleman F, Nollet F, Van Roy FM, Mareel MM 1998 Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient? Cancer Genet Cytogenet. 107(1):76-9. PMID: 9809040. This has been confirmed at ECACC by STR profiling.

REFERENCIA Nº: ECACC Nº: 91091005 (lote No05K025) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Human colon carcinoma

MORFOLOGÍA similar a epitelial

MEDIO DE CULTIVO: McCoy's 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

NUMERO DE PASE:

CARIOTIPO: Modal no. 45

DNA PROFILE: STR-PCR Data: 

Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 10,12
D16S539: 11,13
D5S818: 10,11
D7S820: 11,12
THO1: 8,9
TPOX: 8
vWA: 17,22

PROCEDIMIENTO DE SUBCULTIVO: Dividir los cultivos semiconfluentes 1:3 a 1:10 sembrando 3x10,000 cells/cm²empleando tripsina/EDTA; 5% CO2; 37°C.

NIVEL DE BIOSEGURIDAD: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: Obtained from ATCC

REFERENCIAS: Cancer Res 1981;41:1751; J Nat Cancer Inst 1982;69:767, Cancer 1995;76:201-209, Exp. Cell Res 1994; 214:215-224 , Cancer Res. 1997; 57:488-494 , Cancer Res. 1998; 58:95-101, Proc. Natl. Acad. Sci USA 93;4816-4820, Proc. Natl. Acad. Sci USA93: 8425-8430, Cancer Res. 1997; 57:3562-3568. Abaan OD, Polley EC, Davis SR, Zhu YJ, Bilke S, Walker RL, Pineda M, Gindin Y, Jiang Y, Reinhold WC, Holbeck SL, Simon RM, Doroshow JH, Pommier Y, Meltzer PS.2013 The exomes of the NCI-60 panel: a genomic resource for cancer biology and systems pharmacology. Cancer Res. 73(14):4372-82. PMID: 23856246.

COMENTARIOS: One of 3 strains of malignant cells isolated from a male with colonic carcinoma. Cells are tumourigenic in nude mice and form colonies on agarose. Growth and plating efficiency are enhanced by using a feeder layer of murine fibroblasts (J.Natl. Cancer Inst.1982; 69:757-771)

REFERENCIA Nº: ECACC Nº: 93021013 (lote1758) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Carcinoma humano de cervix

MORFOLOGÍA Epitelial

MEDIO DE CULTIVO: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

NUMERO DE PASE:

CARIOTIPO: 2n = 46 Aneuploide

PROCEDIMIENTO DE SUBCULTIVO: Dividir los cultivos confluentes de 1:2 a 1.6 sembrando 2-4 x 10000 células/cm2 usando tripsina al 0.25% o EDTA/tripsina. Incubar a 37 C y 5% de CO2.

NIVEL DE BIOSEGURIDAD: 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

DEPOSITOR: Obtained from ATCC

REFERENCIAS: Cancer Res 1952;12:264; Proc Soc Exp Biol Med 1954;87:480

COMENTARIOS: Derived from a cervical carcinoma from a 31 year old female. This was the first aneuploid line derived from human tissue maintained in continuous cell culture. Susceptible to Poliovirus type I and adenovirus type 3. Identified as a contaminant in many other cell lines. The cells should be handled under laboratory containment level 2. Ethnicity: Black.

Susceptible a Poliovirus 1 y Ad 3

NOMBRE: REFERENCIA Nº: ECACC Nº: 86062703 (lote No03K004) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

This material is cited in a US and/or other Patent and may not be used to infringe parent claims

DESCRIPCION CELULAR: Human hepatocyte carcinoma

MORFOLOGÍA Epithelial, Adherent

MEDIO DE CULTIVO: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

NUMERO DE PASE:

CARIOTIPO: 2n = 46, modal no. 60

DNA PROFILE: STR-PCR Data:

Amelogenin: X
CSF1PO: 8
D13S317: 12,14
D16S539: 10
D5S818: 13
D7S820: 8,10
THO1: 6,7
TPOX: 9
vWA: 17

PROCEDIMIENTO DE SUBCULTIVO: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

NIVEL DE BIOSEGURIDAD: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet

DEPOSITOR: Mr C B Morris, EMBL, Heidelburg, GERMANY

REFERENCIAS: Nature 1979;282:615; Science 1980;209:497

COMENTARIOS: Derived from an 8 year old male. Cells contain integrated Hepatitis B virus genome. However there is currently no evidence that this cell line produces infectious Hepatitis B virus. The cells should be handled under laboratory containment level 2. Ethnicity: Black

REFERENCIA Nº: ECACC Nº: 86030501 (lote nº 1695) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: carcinoma de cérvix humano

MORFOLOGÍA: Epitelial

MEDIO DE CULTIVO: EMEM (EBSS) + 2 mM GLUTAMINA + 1% de aminoácidos no esenciales + 1mM Piruvato sódico + 10% Suero bovino fetal.

NUMERO DE PASE:

CARIOTIPO: Modal no. 14

PROCEDIMIENTO DE SUBCULTIVO: Dividir los cultivos subconfluentes en 1:3 a 1:6 sembrando de 2-4x10000 células/cm2 empleando tripsina al 0.25% o tripsina/EDTA. Se incuban a 37 C y 5% de CO2. Durante los subcultivos rutinarios las células deben siempre subcultivarse antes de alcanzar la confluencia.

NIVEL DE BIOSEGURIDAD: 1

REFERENCIAS: Cancer Res 1954;14:660; Cancer Res 1955;15:598; Soc Exp Biol Med 1956;193:107

COMENTARIOS:  Originated from tumours produced in irradiated-cortisonised weanling rats after injecting with epidermoid carcinoma tissue from the larynx of a 56 year old male. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

REFERENCIA Nº: ECACC Nº: 85011430  (lote CB No2440) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Human Caucasian hepatocyte carcinoma

MORFOLOGÍA Epithelial Adherent

MEDIO DE CULTIVO: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

NUMERO DE PASE:

CARIOTIPO: Modal no. 55

DNA PROFILE: STR-PCR Data:

Amelogenin:X,Y
CSF1PO:10,11
D13S317:9,13
D16S539:12,13
D5S818:11,12
D7S820:10
THO1:9
TPOX:8,9
vWA: 17

PROCEDIMIENTO DE SUBCULTIVO: Dividir los cultivos confluentes de 1:2 a 1.6 sembrando 2-4 x 10000 células/cm2 usando tripsina al 0.25% o EDTA/tripsina. Incubar a 37 C y 5% de CO2.Pueden crecer formando islotes.

 Requires 5% DMSO and 95% foetal bovine serum (FBS) as cryoprotectant. Growing orders are recommended due to difficulties that can be experienced during the initial start-up of this cell line. Replacements will be charged at full cost where claims cannot be substantiated

NIVEL DE BIOSEGURIDAD: 2. Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

DEPOSITOR: Prof B Knowles, Wistar Institute, Philadelphia

REFERENCIAS Nature 1979;282:615; Science 1980;209:497; In Vitro Cell Dev Biol 1987;23:349

COMENTARIOS: The Hep G2 cell line has been isolated from a liver biopsy of a male Caucasian aged 15 years, with a well differentiated hepatocellular carcinoma. The cells secrete a variety of major plasma proteins e.g. albumin, alpha2-macroglobulin, alpha 1-antitrypsin, transferrin and plasminogen. They have been grown successfully in large scale cultivation systems. Hepatitis B virus surface antigens have not been detected. The cells will respond to stimulation with human growth hormone.

REFERENCIA Nº: ECACC Nº: 98070106 (lote) SUMINISTRADA POR EL BANCO DE CÉLULAS DEL CIC DE LA UNIVERSIDAD DE GRANADA. Se ruega que en las publicaciones derivadas del uso de esta línea celular, se incluya la procedencia citada anteriormente. Estas células son distribuidas para su uso en investigación solamente. No está permitida su distribución con usos comerciales. No se aconseja la distribución a tercenas personas pues de esta práctica surgen la extensión de las líneas celulares contaminadas. Se ruega cumplan las normas establecidas por el banco de células de su referencia, las cuales pueden consultarlas en su página web.

DESCRIPCION CELULAR: Human Caucasian promyelocytic leukaemia

MORFOLOGÍA Lymphoblast

MEDIO DE CULTIVO: RPMI 1640 + 2mM Glutamine + 10-20% Foetal Bovine Serum (FBS).

NUMERO DE PASE:

CARIOTIPO: Modal no. 46, pseudodiploid

PROCEDIMIENTO DE SUBCULTIVO:.. Esta línea cellular crece en suspensión. Centrifugar las células a baja velocidad. 100–150 x g por un máximo de 5 minutos. Quitar el sobrenadante y resuspender a una densidad de 3–5 x 100,000 cells/ml en  medio containing 10% de suero. Incubar a 37 C; 5% CO2.

Cell growth after resuscitation is slow, it may take up to 10 days for proliferation to be established. Check daily. Once the culture is established the serum concentration can be reduced to 10%. Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO2; 37 C; at low density or they may differentiate. At ECACC it has been found to be difficult to recover cells of acceptable viability after freezing in 10% glycerol/90% FBS. We recommend using 10% DMSO/90% FBS for this purpose, but spinning out the cells at resuscitation as above to remove the DMSO as it can cause cells to differentiate. After 6 weeks in culture cells may differentiate.

NIVEL DE BIOSEGURIDAD: 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

DEPOSITOR: Dr Chris Bunce, Department of Medicine, University of Birmingham, UK

REFERENCIAS: Collins et al., (1977) Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture. Nature 270:347–349.

Abaan OD, Polley EC, Davis SR, Zhu YJ, Bilke S, Walker RL, Pineda M, Gindin Y, Jiang Y, Reinhold WC, Holbeck SL, Simon RM, Doroshow JH, Pommier Y, Meltzer PS.2013 The exomes of the NCI-60 panel: a genomic resource for cancer biology and systems pharmacology. Cancer Res. 73(14):4372-82. PMID: 23856246.

COMENTARIOS: The HL-60 cell line was derived from peripheral blood leukocytes obtained by leukopheresis of a 36-year-old Caucasian female with acute promyelocytic leukemia. It was among the first long-term suspension cultures of human myeloid leukaemic cells to be established. Approximately 10% of HL-60 cells spontaneously differentiate and differentiation can be stimulated by polar planar compounds butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid.